Welcome to the Bacillus Genetic Stock Center (BGSC) web site.
Our primary mission is to maintain genetically characterized strains, cloning vectors, and bacteriophage for the genus Bacillus and related organisms and to distribute these materials without prejudice to qualified scientists and educators throughout the world. Since 1978, the National Science Foundation has funded the activities of the BGSC. The Department of Microbiology in the College of Arts and Sciences at The Ohio State University provides facilities and administrative support. The Director of the BGSC is Dr. Daniel R. Zeigler.
pLIKE vector sequences
Over the past two years, there has been considerable interest in the pLIKE vectors from the Thorsten Mascher lab at Ludwig-Maximilians-University (LMU) Munich. These expression vectors feature a bacitracin-inducible promoter, available both in a replicative plasmid (our ECE255) and an amyE-integration vector (ECE256). For the convenience of our users, we have uploaded maps and DNA sequences of these plasmids onto our website. You can download them here. Thanks to Prof. Mascher for providing this document.