Welcome to the Bacillus Genetic Stock Center (BGSC) web site.
Our primary mission is to maintain genetically characterized strains, cloning vectors, and bacteriophage for the genus Bacillus and related organisms and to distribute these materials without prejudice to qualified scientists and educators throughout the world. Since 1978, the National Science Foundation has funded the activities of the BGSC. The Department of Microbiology in the College of Arts and Sciences at The Ohio State University provides facilities and administrative support. The Director of the BGSC is Dr. Daniel R. Zeigler.
B. subtilis essential gene knockdown library
The BGSC is excited to announce the availability of a new collection of Bacillus subtilis 168 mutants designed to explore the functions of 289 essential genes in this organism. The paper describing the construction of this library and its initial characterization will be released online today (26 May 2016) and will appear in the June 2 edition of Cell. The paper is a collaboration among labs at the University of California, San Francisco, Stanford University, University of California, Berkeley, and McMaster University, Hamilton, Ontario. The co-first authors are Jason M. Peters of UCSF and Alexandre Colavin and Handuo Shi of Stanford.
The library uses a CRISPR interference