Welcome to the Bacillus Genetic Stock Center (BGSC) web site.
Our primary mission is to maintain genetically characterized strains, cloning vectors, and bacteriophage for the genus Bacillus and related organisms and to distribute these materials without prejudice to qualified scientists and educators throughout the world. Since 1978, the National Science Foundation has funded the activities of the BGSC. The Department of Microbiology in the College of Arts and Sciences at The Ohio State University provides facilities and administrative support. The Director of the BGSC is Dr. Daniel R. Zeigler.
Standardized, reusable B. subtilis tools
The BGSC is pleased to announce the acquisition of a toolbox of standardized, reusable building blocks for Bacillus subtilis genetics. The Thorsten Mascher lab at Ludwig-Maximilians-Universität München, Germany, has constructed several vector tools following the BioBrick standard. Full details are available in the cited reference, including sequence files in the online supporting material. For convenience, I am including a brief description of these tools.
First, the Bacillus BioBrick Box includes five integration vectors. Each vector contains flanking homology regions for integration into the B. subtilis chromosome; a resistance cassette for selection of integrants; and a